NSUN2 Antibody - C-terminal region : FITC (ARP48811_P050-FITC)

Product Specifications

Gene Name

NOP2/Sun domain family, member 2

Gene Aliases

MISU, MRT5, SAKI, TRM4

Gene ID

54888

Swiss Prot

Q08J23

Accession Number

NP_060225

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human NSUN2

Target

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 is a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) .Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu (CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu (CAA) (Brzezicha et al., 2006 [PubMed 17071714]) .[supplied by OMIM].

Partner Proteins

SUMO2; SUMO3; UBC; SUMO1; NEDD8; TP53RK; PUS1; EFTUD1; XPO5; UGGT1; OSGEP; PANK4; THG1L; SH3GLB1; KIAA1279; UBXN7; TTLL12; ARPC2; ACTR2; ACTR3; FUBP1; TSN; SH3GL1; RPA1; RDX; RANGAP1; PPP2CB; PFDN5; PFDN1; NRD1; PPP1R12A; HARS; GTF2E1; CALU; CALR; UBD; VC

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 93%; Rat: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.