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MRPL15 Antibody - C-terminal region : HRP (ARP46192_P050-HRP)

Selected Variant

ARP46192_P050-HRP

Size: 100 µL

Product Specifications

Gene Name

Mitochondrial ribosomal protein L15

Gene Aliases

L15mt, RPML7, MRP-L7, HSPC145, MRP-L15

Gene ID

29088

Swiss Prot

Q9P015

Accession Number

NP_054894

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human MRPL15

Target

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRPL15 belongs to the ribosomal protein L15P family. It is a 39S subunit protein that belongs to the EcoL15 ribosomal protein family.Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL15 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 15q.

Partner Proteins

CCNDBP1; RUNDC3A; UBC; SOX2; MRPL55; MRPL10; MRPL45; TMEM177; MRPL57; MRPL9; MRPL11; MRPL32; MRPL38; MRPL40; MRPL14; MARC1; NDUFA12; MRPL16; MRPL50; MRPL39; MRPL4; MRPL13; FKBP8; SCFD1; MRPL19; EIF3C; MRPL12; OGDH; NDUFS4; ILF3; DLST; CYC1; COX15; CAND1

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB

Purification

Affinity Purified

Sample Type

MRPL15 is supported by BioGPS gene expression data to be expressed in MCF7

Concentration

0.5 mg/ml

Homology

Cow: 92%; Dog: 100%; Guinea Pig: 79%; Horse: 86%; Human: 100%; Mouse: 100%; Pig: 100%; Rabbit: 100%; Rat: 86%; Zebrafish: 77%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

33kDa

References & Citations

Mehrle, A., Nucleic Acids Res. 34 (DATABASE ISSUE), D415-D418 (2006)

Shipping Conditions

Wet Ice

Protein Length

296

NCBI Gene Symbol

MRPL15

NCBI GB Accession Number

MRPL15

Host or Source

Rabbit

Protein Name

39S ribosomal protein L15, mitochondrial

Nucleotide Accession Number

NM_014175

Peptide Sequence

KDELFKMLCTRKDPRQIFFGLAPGWVVNMADKKILKPTDENLLKYYTS

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