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PRIM1 Antibody - N-terminal region : HRP (ARP46013_T100-HRP)

Product Specifications

Gene Name

Primase, DNA, polypeptide 1 (49kDa)

Gene Aliases

P49

Gene ID

5557

Swiss Prot

P49642

Accession Number

NP_000937

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human PRIM1

Target

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.

Partner Proteins

FAM96B; CIAO1; UBC; MMS19; MDC1; POLA2; PRIM2; POLE; POLA1; COPS6; RPA3; RPA2; RPA1; RAE1; MRPL38; TP53; XRCC5

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 93%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Yeast: 80%; Zebrafish: 79%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

50kDa

References & Citations

Arezi, B., (1999) Biochemistry 38 (39), 12899-12907

Shipping Conditions

Wet Ice

Product Datasheet

Anti-PRIM1 (ARP46013_T100-HRP) antibody

Protein Length

420

NCBI Gene Symbol

PRIM1

NCBI GB Accession Number

PRIM1

Host or Source

Rabbit

Protein Name

DNA primase small subunit

Nucleotide Accession Number

NM_000946

Peptide Sequence

SQYYRWLNYGGVIKNYFQHREFSFTLKDDIYIRYQSFNNQSDLEKEMQKM

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