Sheep anti Human chk 1 kinase
Product Specifications
Background
One mechanism by which checkpoints maintain the fidelity of cell cycle events is through blocking mitosis in response to unreplicated or damaged DNA. In most species this occurs through inhibiting activation of the cyclic-dependent kinase cdc2, which regulates entry in to mitosis. Chk1, a kinase involved in the DNA damage checkpoint response can phosphorylate Cdc25C an activator of cdc2. It is hypothesized that chk 1 induces serine 216 phosphorylation of Cdc 25C and subsequent binding negatively regulated Cdc25C, thus preventing it from activating cdc2.
Synonyms
Sheep Anti Human chk 1 kinase, checkpoint kinase 1
UniProt
O14757
Host
Sheep
Species Reactivity
Human,Mouse,Rat,Monkey
Isotype
IgG
Conjugation
Unconjugated
Type
Polyclonal Antibody
Source
Sheep were immunized with a protein corresponding to amino acid sequence 1-474 of Human chk-1, having a molecular weight of 54 kDa.
Applications
WB, IP
Field of Research
Signal Transduction
Purification Method
Ammonium Sulfate Precipitation
Assay Principle
Western Blot at 1 – 10 µg/mL.
Stability
See expiration date on vial
Concentration
See vial for Concentration
Form
Provided as solution in phosphate buffered saline with 0,08% sodium azide
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
References & Citations
1. Wang, Y., et al. 'CHK1 kinase activity assay.' Methods Mol Biol 2004, 281, 143-151._x000D_
2. Yarden, R.I., et al. 'BRCA1 regulates the G2/M checkpoint by activating Chk1 kinase upon DNA damage.' Nat Genet 2002, 30, 285-289_x000D_
3. Nakajo, N., et al. 'Involvement of Chk1 kinase in prophase I arrest of Xenopus oocytes.' Dev Biol 1999, 207, 432-444
Shipping Conditions
Ambient Temperature
Storage Conditions
Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles
Functional Analysis
WB
CAS Number
9007-83-4
Curated Selection
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