CDC27 Antibody - middle region : HRP (ARP43603_P050-HRP)

Product Specifications

Gene Name

Cell division cycle 27 homolog (S. cerevisiae)

Gene Aliases

APC3, HNUC, NUC2, H-NUC, ANAPC3, CDC27Hs, D0S1430E, D17S978E

Gene ID

996

Swiss Prot

P30260

Accession Number

NP_001247

Host

Rabbit

Reactivity

Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human CDC27

Target

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Partner Proteins

ANAPC2; UBE2S; PTTG1; FBXO43; MAD2L1; COMT; CDC20; CCNB1; BUB1B; UBC; BIRC6; ANAPC4; CDH1; FZR1; MIB1; HECW2; ANAPC11; PHF8; CUEDC2; Sirt2; Dlg4; GKN1; ANAPC7; ANAPC5; DCPS; ADNP; MDC1; PTEN; TRIM33; REV1; ANAPC15; VCP; MAD2L2; NOTCH1; PAXIP1; UBE4B; TTK

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

Purification

Affinity Purified

Sample Type

CDC27 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Rabbit: 100%; Rat: 100%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.