CDC27 Antibody - middle region: FITC (ARP43603_P050-FITC)
Product Specifications
Gene Name
Cell division cycle 27 homolog (S. cerevisiae)
Gene Aliases
APC3, HNUC, NUC2, H-NUC, ANAPC3, CDC27Hs, D0S1430E, D17S978E
Gene ID
996
Swiss Prot
P30260
Accession Number
NP_001247
Host
Rabbit
Reactivity
Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human CDC27
Target
The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and the gene product of Schizosaccharomyces pombe nuc 2. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eucaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and thus may be involved in controlling the timing of mitosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Partner Proteins
ANAPC2; UBE2S; PTTG1; FBXO43; MAD2L1; COMT; CDC20; CCNB1; BUB1B; UBC; BIRC6; ANAPC4; CDH1; FZR1; MIB1; HECW2; ANAPC11; PHF8; CUEDC2; Sirt2; Dlg4; GKN1; ANAPC7; ANAPC5; DCPS; ADNP; MDC1; PTEN; TRIM33; REV1; ANAPC15; VCP; MAD2L2; NOTCH1; PAXIP1; UBE4B; TTK
Clonality
Polyclonal
Conjugation
FITC: Fluorescein Isothiocyanate
Type
Polyclonal Antibody
Applications
WB
Purification
Affinity Purified
Sample Type
CDC27 is strongly supported by BioGPS gene expression data to be expressed in NCI-H226
Concentration
0.5 mg/mL
Homology
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Rabbit: 100%; Rat: 100%
Format
Liquid. Purified antibody supplied in 1x PBS buffer.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
92kDa
Shipping Conditions
Wet Ice
Protein Length
824
NCBI Gene Symbol
CDC27
Protein Name
Cell division cycle protein 27 homolog
Nucleotide Accession Number
NM_001256
Curated Selection
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