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RNF25 Antibody - middle region : HRP (ARP43300_P050-HRP)

Product Specifications

Gene Name

Ring finger protein 25

Gene Aliases

AO7

Gene ID

64320

Swiss Prot

Q96BH1

Accession Number

NP_071898

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human RNF25

Target

RNF25 contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.The protein encoded by this gene contains a RING finger motif. The mouse counterpart of this protein has been shown to interact with Rela, the p65 subunit of NF-kappaB (NFKB), and modulate NFKB-mediated transcription activity. The mouse protein also binds ubiquitin-conjugating enzymes (E2s) and is a substrate for E2-dependent ubiquitination.

Partner Proteins

UBE2E3; UBE2D2; UBC; TRIM27; UBE2D1; UBE2U; UBE2D4; UBE2L6; UBE2E2; UBE2E1; UBE2D3; RELA; NKD2

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC, WB

Purification

Affinity Purified

Sample Type

RNF25 is supported by BioGPS gene expression data to be expressed in HepG2

Concentration

0.5 mg/ml

Homology

Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 86%; Human: 100%; Mouse: 100%; Rabbit: 92%; Rat: 100%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

51kDa

References & Citations

Asamitsu, K., (2003) J. Biol. Chem. 278 (29), 26879-26887

Shipping Conditions

Wet Ice

Protein Length

459

NCBI Gene Symbol

RNF25

NCBI GB Accession Number

RNF25

Host or Source

Rabbit

Protein Name

E3 ubiquitin-protein ligase RNF25

Nucleotide Accession Number

NM_022453

Peptide Sequence

CREPLVYDLASLKAAPEPQQPMELYQPSAESLRQQEERKRLYQRQQERGG

Frequently Asked Questions

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