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CHFR Antibody - N-terminal region : HRP (ARP43250_P050-HRP)

Product Specifications

Gene Name

Checkpoint with forkhead and ring finger domains, E3 ubiquitin protein ligase

Gene Aliases

RNF116, RNF196

Gene ID

55743

Swiss Prot

Q96EP1

Accession Number

NP_060693

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human CHFR

Target

CHFR is an E3 ubiquitin-protein ligase required to transiently arrest cells in early prophase when they are exposed to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome moves apart from each other along the periphery of the nucleus. CHFR probably promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBC13-MMS2 (UBE2N-UBE2V2) heterodimer. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation, but are rather involved in signaling cellular stress. This suggests that it may be involved in signaling the presence of mitotic stress caused by microtubule poisons.

Partner Proteins

USP7; VIM; UBE2D2; AURKA; HLTF; RANBP2; PLK1; PCNA; MCM2; HDAC2; HDAC1; CDK5; CDC20; PARP1; SIN3A; CHFR; UBC13; SUMO1; UBE2I; LRP1; UBE2V1; UBE2N; UBE2D1; TRIM9; RNF125; CBLC; RNF7; RELA; RNF8; HIST2H2BE; HIST2H2AC; UBC; SMARCD1; SMARCB1; SMARCA4; KIF22

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC, WB

Purification

Affinity Purified

Sample Type

CHFR is supported by BioGPS gene expression data to be expressed in HepG2

Concentration

0.5 mg/ml

Homology

Cow: 93%; Dog: 93%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

69kDa

References & Citations

Kang, H.C., Cancer Lett. 260 (1-2), 170-179 (2008)

Shipping Conditions

Wet Ice

Product Datasheet

Anti-CHFR (ARP43250_P050-HRP) antibody

Protein Length

623

NCBI Gene Symbol

CHFR

NCBI GB Accession Number

CHFR

Host or Source

Rabbit

Protein Name

E3 ubiquitin-protein ligase CHFR

Nucleotide Accession Number

NM_018223

Peptide Sequence

REWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVIN

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