NEDD4L Antibody - middle region : Biotin (ARP43192_P050-Biotin)

Product Specifications

Gene Name

Neural precursor cell expressed, developmentally down-regulated 4-like, E3 ubiquitin protein ligase

Gene Aliases

RSP5, PVNH7, NEDD4-2, NEDD4.2, hNEDD4-2

Gene ID

23327

Swiss Prot

Q96PU5-5

Accession Number

NP_056092

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human NEDD4L

Target

NEDD4L is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. NEDD4L inhibits TGF-beta signaling by triggering SMAD2 and TGFR1 ubiquitination and proteasome-dependent degradation. NEDD4L promotes ubiquitination and internalization of various plasma membrane channels such as ENaC, Nav1.2, Nav1.3, Nav1.5, Nav1.7, Nav1.8, Kv1.3, EAAT1 or CLC5. NEDD4L also promotes ubiquitination and degradation of SGK.

Partner Proteins

NEDD4L; DAZAP2; UBE2D1; PTCH1; WNK3; TNK2; SNCA; SLC9A3; SMAD6; KCNH2; HCN1; SMAD3; UBE2N; SNA3; NOTCH1; UBE2D3; UBC; SGK1; KCNQ1; SGK3; GORAB; MED28; FBXL15; vpr; RNF11; AATF; YWHAQ; LAPTM5; SCNN1B; SMAD2; ARRDC3; ARRDC4; UBE2L3; UBE2D2; SMAD7; UBE2K; UB

Clonality

Polyclonal

Conjugation

Biotin

Type

Polyclonal Antibody

Applications

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.