SF3B3 Antibody - middle region : Biotin (ARP40730_P050-Biotin)

Product Specifications

Gene Name

Splicing factor 3b, subunit 3, 130kDa

Gene Aliases

RSE1, SAP130, SF3b130, STAF130

Gene ID

23450

Swiss Prot

Q15393

Accession Number

NP_036558

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human SF3B3

Target

SF3B3 is subunit 3 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 3 has also been identified as a component of the STAGA (SPT3-TAF (II) 31-GCN5L acetylase) transcription coactivator-HAT (histone acetyltransferase) complex, and the TFTC (TATA-binding-protein-free TAF (II) -containing complex) . These complexes may function in chromatin modification, transcription, splicing, and DNA repair.

Partner Proteins

HUWE1; UBC; FUS; SUMO2; NUDCD3; LGR4; SUMO1; NEDD8; MDM2; RPA3; RPA2; RPA1; ERG; SUZ12; EED; RNF2; EZH2; PRPF40A; WWOX; APBB1; WBP4; TCERG1; FBXO6; CUL1; CUL2; CUL3; CUL4A; SKP2; SKP1; CD81; IGSF8; RBM6; EFTUD2; WHSC1; IFIT3; HSP90AA1; HSPB1; EPS8; TOE1

Clonality

Polyclonal

Conjugation

Biotin

Type

Polyclonal Antibody

Applications

Purification

Affinity Purified

Sample Type

SF3B3 is supported by BioGPS gene expression data to be expressed in HCT15

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.