CPSF4 Antibody - C-terminal region : Biotin (ARP40675_P050-Biotin)

Product Specifications

Gene Name

Cleavage and polyadenylation specific factor 4, 30kDa

Gene Aliases

NAR, NEB1, NEB-1, CPSF30

Gene ID

10898

Swiss Prot

O95639

Accession Number

EAW76662

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human CPSF4

Target

Inhibition of the nuclear export of poly (A) -containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs.

Partner Proteins

FIP1L1; RBBP6; MEOX2; CLK2; NPM1; MDC1; ECT2; UBC; CSTF2T; RRAGA; CDC73; SLC12A9; MARK3

Clonality

Polyclonal

Conjugation

Biotin

Type

Polyclonal Antibody

Applications

Purification

Affinity Purified

Sample Type

CPSF4 is supported by BioGPS gene expression data to be expressed in Jurkat

Concentration

0.5 mg/ml

Homology

Cow: 93%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.