PCBP2 Antibody - middle region : HRP (ARP40568_T100-HRP)

Product Specifications

Gene Name

Poly (rC) binding protein 2

Gene Aliases

HNRPE2, HNRNPE2, hnRNP-E2

Gene ID

5094

Swiss Prot

Q6IPF4

Accession Number

NP_005007

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Guinea Pig, Horse

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human PCBP2

Target

PCBP2 appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly (rC) -binding proteins. This protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes.The protein encoded by this gene appears to be multifunctional. It along with PCBP-1 and hnRNPK corresponds to the major cellular poly (rC) -binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-1 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability. This multiexon structural mRNA is thought to be retrotransposed to generate PCBP-1 intronless gene which has similar functions. This gene and PCBP-1 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. It also has two processed pseudogenes PCBP2P1 and PCBP2P2. There are presently two alternatively spliced transcript variants described for this gene.

Partner Proteins

FUS; HUWE1; UBC; SNRPA; SUMO2; SUMO3; CEP250; MDM2; EED; TARDBP; ADRB2; IGSF8; HMGA1; CD81; HMGA2; WDR83; SLU7; CASK; UBL4A; VCAM1; PCBP2; NOS2; ITGA4; FN1; BRCA1; PRPF40A; SF3B2; NKAP; WDR77; PAXIP1; BARD1; PPIB; EEF1A1; FBXO6; UBD; PCBP1; QKI; CDK2; COP

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC, WB

Sample Type

There is BioGPS gene expression data showing that PCBP2 is expressed in Jurkat

Concentration

0.5 mg/ml

Homology

Cow: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.