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SF3B1 Antibody - middle region : HRP (ARP40248_T100-HRP)

Product Specifications

Gene Name

Splicing factor 3b, subunit 1, 155kDa

Gene Aliases

MDS, PRP10, Hsh155, PRPF10, SAP155, SF3b155

Gene ID

23451

Swiss Prot

O75533

Accession Number

AAH56155

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human SF3B1

Target

SF3B1 is the subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP) . The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome.

Partner Proteins

SART3; SUMO2; SUMO3; MDM2; STAU1; UBC; LGR4; WWOX; RPA3; RPA2; RPA1; ERG; EED; RNF2; EZH2; SUZ12; APBB1; PRPF40A; WBP4; TCERG1; FBXO6; RBM17; SNIP1; SF3B6; WHSC1; RPS2; RPL23A; RPL18A; RPL10; RPL3; PLRG1; SMAD9; SMAD5; SMAD1; IL7R; FN1; ESR1; CSNK2A1; EIF

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC, WB

Sample Type

SF3B1 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Goat: 86%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

87kDa

References & Citations

Strausberg, R.L., (2002) Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903

Shipping Conditions

Wet Ice

Protein Length

789

NCBI Gene Symbol

SF3B1

NCBI GB Accession Number

SF3B1

Host or Source

Rabbit

Protein Name

Splicing factor 3B subunit 1

Nucleotide Accession Number

NM_001005526

Peptide Sequence

LLNDIPQSTEQYDPFAEHRPPKIADREDEYKKHRRTMIISPERLDPFADG

Subunit

1

Frequently Asked Questions

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