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MED21 Antibody - middle region : HRP (ARP37735_P050-HRP)

Selected Variant

ARP37735_P050-HRP

Size: 100 µL

Product Specifications

Gene Name

Mediator complex subunit 21

Gene Aliases

SRB7, SURB7, hSrb7

Gene ID

9412

Swiss Prot

Q13503

Accession Number

NP_004255

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human MED21

Target

MED21 belongs to the Mediator complex subunit 21 family. It is the component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.

Partner Proteins

MAGEB4; SSC5D; ZNF655; BLOC1S6; MED21; CDK8; MED19; MED26; UBC; HTT; FBXW7; MED11; MED8; MED28; MED29; MED18; MED4; MED16; MED6; MED13; MED12; MED24; MED17; MED14; MED22; MED1; ELAVL1; VAV2; SKP1; THRA; THRB; MED10; TP53; SREBF1; ZC3H13; ZSCAN1; PCBD2; TA

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rat: 100%; Zebrafish: 100%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

15kDa

References & Citations

Andersen, J.S., (2005) Nature 433 (7021), 77-83

Shipping Conditions

Wet Ice

Protein Length

144

NCBI Gene Symbol

MED21

NCBI GB Accession Number

MED21

Host or Source

Rabbit

Protein Name

Mediator of RNA polymerase II transcription subunit 21

Nucleotide Accession Number

NM_004264

Peptide Sequence

DSLPSEESTAALQAASLYKLEEENHEAATCLEDVVYRGDMLLEKIQSALA

Subunit

21

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