MCM4 Antibody - C-terminal region : HRP (ARP36663_T100-HRP)

Product Specifications

Gene Name

Minichromosome maintenance complex component 4

Gene Aliases

NKCD, CDC21, CDC54, IMD54, NKGCD, hCdc21, P1-CDC21

Gene ID

4173

Swiss Prot

P33991

Accession Number

NP_877423

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human MCM4

Target

The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcript variants encoding the same protein have been reported.

Partner Proteins

HUWE1; UBC; PCNA; SUMO2; FKBP5; MCMBP; MDM2; POLK; CLUH; NCKAP1; CAP1; CAP2; EIF3B; MCM2; KPNA3; PRMT1; FKBP4; MCM7; MCM6; FBXW4; FBXO46; ITGA4; CDC5L; gag-pol; ORC6; CCNA1; VCAM1; DBF4; CDC7; LIG4; MCM5; MCM3; DCTN2; SF3A1; UBD; NEDD8; TONSL; SIRT7; ASF1

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

Sample Type

MCM4 is strongly supported by BioGPS gene expression data to be expressed in Jurkat

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 93%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 86%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.