MCM10 Antibody - middle region : FITC (ARP36656_P050-FITC)

Product Specifications

Gene Name

Minichromosome maintenance complex component 10

Gene Aliases

CNA43, DNA43, PRO2249

Gene ID

55388

Swiss Prot

B7ZKZ8

Accession Number

NP_060988

Host

Rabbit

Reactivity

Human, Cow, Dog, Horse, Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human MCM10

Target

This protein is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding.The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2. It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner. Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Partner Proteins

GOLGA2; MCM6; TRIM37; CCND3; ORC3; CDC45; CDC7; UBC; ORC4; ORC2; MCM7; MCM3; MCM2; CDKN2A; CDKN1A; CDK6; CDC6; CDC5L; CCND1; APP; RBX1; VPRBP; CUL4A; DDB1; IKBKG; RPAP1; CEP70; HAUS1; CCDC85B; DPPA2; NINL; ZBTB8B; HOOK2; MCM4; ZBTB43; CEP72; CALCOCO2; KRT

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

Purification

Affinity Purified

Sample Type

MCM10 is supported by BioGPS gene expression data to be expressed in Jurkat

Concentration

0.5 mg/ml

Homology

Cow: 77%; Dog: 86%; Horse: 93%; Human: 100%; Pig: 77%; Rabbit: 86%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.