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DDX50 Antibody - N-terminal region : FITC (ARP36496_P050-FITC)

Selected Variant

ARP36496_P050-FITC

Size: 100 µL

Product Specifications

Gene Name

DEAD (Asp-Glu-Ala-Asp) box polypeptide 50

Gene Aliases

GU2, GUB, mcdrh, RH-II/GuB

Gene ID

79009

Swiss Prot

Q9BQ39

Accession Number

NP_076950

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Guinea Pig, Horse, Pig

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human DDX50

Target

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. DDX50 is a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box enzyme that may be involved in ribosomal RNA synthesis or processing. This gene and DDX21, also called RH-II/GuA, have similar genomic structures and are in tandem orientation on chromosome 10, suggesting that the two genes arose by gene duplication in evolution. This gene has pseudogenes on chromosomes 2, 3 and 4. Alternative splicing of this gene generates multiple transcript variants, but the full length nature of all the other variants but one has not been defined.

Partner Proteins

SUMO3; UBC; LIN28A; PRKRA; TARBP2; EED; RNF2; TARDBP; SRPK2; WHSC1; KRAS; ESR1; BARD1; CAND1; CUL3; HDGF; STAU1; SRRM2

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

WB

Purification

Affinity Purified

Sample Type

DDX50 is supported by BioGPS gene expression data to be expressed in HEK293T

Concentration

0.5 mg/ml

Homology

Cow: 86%; Guinea Pig: 77%; Horse: 86%; Human: 100%; Mouse: 93%; Pig: 86%; Rat: 92%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

82kDa

References & Citations

Olsen, J.V., (2006) Cell 127 (3), 635-648

Shipping Conditions

Wet Ice

Protein Length

737

NCBI Gene Symbol

DDX50

NCBI GB Accession Number

DDX50

Host or Source

Rabbit

Protein Name

ATP-dependent RNA helicase DDX50

Nucleotide Accession Number

NM_024045

Peptide Sequence

PGKLLWGDIMELEAPLEESESQKKERQKSDRRKSRHHYDSDEKSETRENG

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