UPF1 Antibody - middle region : Biotin (ARP36350_P050-Biotin)

Product Specifications

Gene Name

UPF1 regulator of nonsense transcripts homolog (yeast)

Gene Aliases

UTF, HUPF1, NORF1, RENT1, smg-2, pNORF1

Gene ID

5976

Swiss Prot

Q92900

Accession Number

NP_002902

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human UPF1

Target

UPF1 is a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD) . When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. UPF1 is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene.This gene encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD) . When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping. Use of multiple polyadenylation sites has been noted for this gene. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Partner Proteins

UBC; SUMO2; STAU1; CDC37; IVNS1ABP; MOV10; DNA2; POP1; LIN28A; UPF3B; STAU2; UPF2; EZH2; BMI1; SUZ12; RNF2; GNPTG; RHOXF2; ACSS2; NADSYN1; PLEKHB2; PLEKHA5; TARDBP; RPRD2; ABHD16A; NDUFB10; DXO; SMG1; RUVBL2; RBM8A; SMG7; EIF4A3; RUVBL1; GSPT2; SMG5; GSPT

Clonality

Polyclonal

Conjugation

Biotin

Type

Polyclonal Antibody

Applications

WB, IHC

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 79%; Horse: 93%; Human: 100%; Mouse: 93%; Rat: 93%; Zebrafish: 83%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.