ENO1 Antibody - middle region : Biotin (ARP34377_P050-Biotin)
Product Specifications
Gene Name
Enolase 1, (alpha)
Gene Aliases
NNE, PPH, MPB1, ENO1L1, HEL-S-17
Gene ID
2023
Swiss Prot
Q53HR3
Accession Number
NP_001419
Host
Rabbit
Reactivity
Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Zebrafish
Immunogen
The immunogen is a synthetic peptide directed towards the middle region of human ENO1
Target
ENO1 encodes one of three enolase isoenzymes found in mammals; it encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.
Partner Proteins
UBC; FUS; HUWE1; ISG15; SUMO2; SUMO3; IVNS1ABP; STAU1; MDM2; WWOX; EED; FBXO6; UPF2; YWHAQ; UBD; TERT; SRC; RAD52; NPM1; BHLHE40; VCAM1; HCVgp1; ITGA4; FN1; ATF2; SERPING1; AMBP; LIG4; BRCA1; MDC1; PGK1; ITPA; GAPDH; FYN; FLNC; ENO3; Htt; TPI1; PPIA; AKT1
Clonality
Polyclonal
Conjugation
Biotin
Type
Polyclonal Antibody
Applications
WB, IHC
Purification
Affinity Purified
Concentration
0.5 mg/ml
Homology
Cow: 79%; Dog: 93%; Guinea Pig: 93%; Horse: 79%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 93%; Rat: 100%; Zebrafish: 93%
Format
Liquid. Purified antibody supplied in 1x PBS buffer.
Reconstitution
All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.
Molecular Weight
47kDa
References & Citations
Lee, K.H., et al., (2003) Arthritis Rheum. 48 (7), 2025-2035
Shipping Conditions
Wet Ice
Protein Length
434
NCBI Gene Symbol
ENO1
NCBI GB Accession Number
ENO1
Host or Source
Rabbit
Protein Name
Alpha-enolase
Nucleotide Accession Number
NM_001428
Peptide Sequence
VAASEFFRSGKYDLDFKSPDDPSRYISPDQLADLYKSFIKDYPVVSIEDP
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