MED27 Antibody - middle region : FITC (ARP33743_P050-FITC)

Product Specifications

Gene Name

Mediator complex subunit 27

Gene Aliases

MED3, CRSP8, CRAP34, CRSP34, TRAP37

Gene ID

9442

Swiss Prot

Q6P2C8

Accession Number

NP_004260

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human MED27

Target

The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. MED27 is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors.

Partner Proteins

UBC; CDK19; CDK8; MED19; ARFGAP1; MED26; SOX5; POLR2C; RBPJ; AFP; FBXW7; EPAS1; MED4; MED12; MED14; MED1; MED28; OBFC1; MED18; ZC3H13; QKI; TRIP4; TADA2A; MED9; MED30; MED8; MED22; MED10; MED29

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

Purification

Affinity Purified

Sample Type

MED27 is strongly supported by BioGPS gene expression data to be expressed in 721_B, HeLa, Jurkat

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 85%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 92%; Rabbit: 85%; Rat: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.