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ALF Antibody - N-terminal region : HRP (ARP32837_P050-HRP)

Selected Variant

ARP32837_P050-HRP

Size: 100 µL

Product Specifications

Gene Name

General transcription factor IIA, 1-like

Gene Aliases

ALF

Gene ID

11036

Swiss Prot

Q9UNN4

Accession Number

NP_751946

Host

Rabbit

Reactivity

Human, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human ALF

Target

The assembly and stability of the RNA polymerase II transcription pre-initiation complex on a eukaryotic core promoter involves the effects of TFIIA on the interaction between TATA-binding protein (TBP) and DNA. ALF is a germ cell-specific counterpart of the large (alpha/beta) subunit of general transcription factor TFIIA that is able to stabilize the binding of TBP to DNA and may be uniquely important to testis biology.

Partner Proteins

GAB1; NKX2-3; ZBTB3; TLX3; MAFF; RBFOX2; HMG20A; PAX9; SIX6; MAFG; HOXC11; HOXC9; HOXA5; TLX2; EMX2; EMX1; CEBPE; ATF4; ASCL3; GTF2A2; SUB1; ID3; CSNK2A2; CSNK2A1; TBP

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Cow: 92%; Dog: 92%; Guinea Pig: 83%; Horse: 100%; Human: 100%; Rabbit: 91%; Rat: 100%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

50kDa

References & Citations

Upadhyaya, A.B., et al., (2002) J. Biol. Chem. 277 (37), 34208-34216

Shipping Conditions

Wet Ice

Protein Length

453

NCBI Gene Symbol

GTF2A1L

NCBI GB Accession Number

GTF2A1L

Host or Source

Rabbit

Protein Name

TFIIA-alpha and beta-like factor

Nucleotide Accession Number

NM_172196

Peptide Sequence

VIPAGRTLPSFTTAELGTSNSSANFTFPGYPIHVPAGVTLQTVSGHLYKV

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