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PCK1 Antibody - middle region : HRP (ARP32801_T100-HRP)

Selected Variant

ARP32801_T100-HRP

Size: 100 µL

Product Specifications

Gene Name

Phosphoenolpyruvate carboxykinase 1 (soluble)

Gene Aliases

PCKDC, PEPCK1, PEPCKC, PEPCK-C

Gene ID

5105

Swiss Prot

P35558

Accession Number

NP_002582

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human PCK1

Target

PCK1 is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of PCK1 can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet.

Partner Proteins

UBC; FBXO25; C1R; APP; CUL5; BAG6; TCP1; TCEB1; SKP1; RPS15A; RPS6; RPS3A; RPS3; RPLP0; RPL4; PSMD12; PSMD2; PSMC5; PSMC4; PSMC2; PSMC1; PSMB7; PSMB4; PSMB1; PSMA5; PSMA2; PCK1; LDHA; HSPD1; HSPA1L; DNAJA1; HLA-C; HLA-B; GNAS; GNAI1; GNA12; FASN; EP300; T

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC, WB

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 100%; Pig: 93%; Rabbit: 86%; Rat: 100%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

69kDa

References & Citations

Hajarnis, S., et al., (2005) J Biol Chem. 280 (31), 28272-80

Shipping Conditions

Wet Ice

Protein Length

622

NCBI Gene Symbol

PCK1

NCBI GB Accession Number

PCK1

Host or Source

Rabbit

Protein Name

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Nucleotide Accession Number

NM_002591

Peptide Sequence

NGFFGVAPGTSVKTNPNAIKTIQKNTIFTNVAETSDGGVYWEGIDEPLAS

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