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CHEK1 Antibody - middle region : HRP (ARP32589_P050-HRP)

Selected Variant

ARP32589_P050-HRP

Size: 100 µL

Product Specifications

Gene Name

Checkpoint kinase 1

Gene Aliases

CHK1

Gene ID

1111

Swiss Prot

O14757

Accession Number

NP_001265

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human CHEK1

Target

CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.

Partner Proteins

RELA; CUL4A; UBC; SETMAR; DDB1; TNRC6B; ATR; HECW2; CLSPN; DTL; BTG3; TOPBP1; MAPKAPK2; HSP90AA1; CDC25C; RAD51; PIGM; CUL1; VCP; CDC25A; ATM; APP; MED15; NEK2; TLK1; RB1; BRCA1; FEM1B; XIAP; COPS5; MEPE; LATS2; TP53; Chek1; MEN1; USP7; DNMT1; FANCE; PCNA

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

IHC, WB

Purification

Affinity Purified

Sample Type

CHEK1 is supported by BioGPS gene expression data to be expressed in HepG2

Concentration

0.5 mg/ml

Homology

Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 79%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

54kDa

References & Citations

Kramer, A., et al., (2004) Nat. Cell Biol. 6 (9), 884-891

Shipping Conditions

Wet Ice

Protein Length

476

NCBI Gene Symbol

CHEK1

NCBI GB Accession Number

CHEK1

Host or Source

Rabbit

Protein Name

Serine/threonine-protein kinase Chk1

Nucleotide Accession Number

NM_001274

Peptide Sequence

WSCGIVLTAMLAGELPWDQPSDSCQEYSDWKEKKTYLNPWKKIDSAPLAL

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