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POLR2B Antibody - middle region : FITC (ARP32456_P050-FITC)

Product Specifications

Gene Name

Polymerase (RNA) II (DNA directed) polypeptide B, 140kDa

Gene Aliases

RPB2, POL2RB, hRPB140

Gene ID

5431

Swiss Prot

P30876

Accession Number

NP_000929

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human POLR2B

Target

POLR2B encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA.

Partner Proteins

ESR1; UBC; TCEB3; SRC; PSMB9; SUMO2; XRN2; WWOX; SUZ12; RNF2; NEDD4; ITCH; RABEP2; HECW2; FBXO6; CDK20; HDAC11; ILK; POLR2C; MED19; MED26; RECQL5; VCP; BARD1; MDC1; POLR3F; TUBB4B; TUBA4A; POLR2I; POLR2E; POLR2D; POLR2A; DCTN1; ATP6V1B2; MEN1; CDK7; CCNH

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

WB

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

134kDa

References & Citations

Zhou, M., et al., (2003) Proc. Natl. Acad. Sci. U.S.A. 100 (22), 12666-12671

Shipping Conditions

Wet Ice

Protein Length

1174

NCBI Gene Symbol

POLR2B

NCBI GB Accession Number

POLR2B

Host or Source

Rabbit

Protein Name

DNA-directed RNA polymerase II subunit RPB2

Nucleotide Accession Number

NM_000938

Peptide Sequence

FFRSVFYRSYKEQESKKGFDQEEVFEKPTRETCQGMRHAIYDKLDDDGLI

Subunit

RPB2

Frequently Asked Questions

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