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SPI1 Antibody - middle region : HRP (ARP31421_P050-HRP)

Product Specifications

Gene Name

Spleen focus forming virus (SFFV) proviral integration oncogene spi1

Gene Aliases

OF, PU.1, SFPI1, SPI-1, SPI-A

Gene ID

6688

Swiss Prot

P17947

Accession Number

NP_003111

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig

Immunogen

The immunogen is a synthetic peptide directed towards the middle region of human SPI1

Target

SPI1 is known to collaborate with multiple transcription factors and to regulate expression of immunoglobulin genes. Lack of SPI1 expression is associated with defective immunoglobulin transcription in Hodgkin and Reed-Sternberg cells of classical Hodgkin disease

Partner Proteins

HOXA10; TMX1; VAV1; IRF1; IRF8; GATA3; GATA2; GATA1; GFI1; TBP; RB1; BYRF1; RARA; PML; HDAC3; SKI; SIN3A; HDAC1; BCL6; CEBPA; PIP; SPIB; SSRP1; IRF4; KAT6A; CEBPE; NFATC1; CREBBP; NONO; CEBPB; JUN; ERG; NFKB1; MITF; FUS; CREM; ATF1; CEBPD; MECP2; DNMT3B

Clonality

Polyclonal

Conjugation

HRP: Horseradish Peroxidase

Type

Polyclonal Antibody

Applications

WB

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Cow: 93%; Dog: 100%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 92%; Pig: 100%; Rat: 100%

Format

Liquid. Purified antibody is supplied in high phosphate PBS, 100 mm phosphate, 150 mM NaCl, pH 7.6.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

30kDa

References & Citations

Lin, M. et al., (2004) Cell. Microbiol. 6 (2), 175-186

Shipping Conditions

Wet Ice

Protein Length

264

NCBI Gene Symbol

SPI1

NCBI GB Accession Number

SPI1

Host or Source

Rabbit

Protein Name

Transcription factor PU.1

Nucleotide Accession Number

NM_003120

Peptide Sequence

HPSLGHQVSYLPRMCLQYPSLSPAQPSSDEEEGERQSPPLEVSDGEADGL

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