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POU1F1 Antibody - N-terminal region : FITC (ARP31418_T100-FITC)

Selected Variant

ARP31418_T100-FITC

Size: 100 µL

Product Specifications

Gene Name

POU class 1 homeobox 1

Gene Aliases

PIT1, CPHD1, GHF-1, Pit-1, POU1F1a

Gene ID

5449

Swiss Prot

P28069

Accession Number

NP_000297

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Goat, Guinea Pig, Horse, Pig, Rabbit, Sheep

Immunogen

The immunogen is a synthetic peptide directed towards the N terminal region of human POU1F1

Target

PIT1 is a pituitary-specific transcription factor responsible for pituitary development and hormone expression in mammals and is a member of the POU family of transcription factors that regulate mammalian development. The POU family is so named because the first 3 members identified were PIT1 and OCT1 of mammals, and Unc-86 of C. elegans. PIT1 contains 2 protein domains, termed POU-specific and POU-homeo, which are both necessary for high affinity DNA binding on genes encoding growth hormone and prolactin. PIT1 is also important for regulation of the genes encoding prolactin and thyroid-stimulating hormone beta subunit by thyrotropin-releasing hormone and cyclic AMP.

Partner Proteins

CEBPD; NCOR1; CREBBP; MED1; GATA2; NR1I3; NR1I2; PITX1; JUN; ETS1; NR3C1; VDR; PPARG; PPARA

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

WB

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 92%; Goat: 100%; Guinea Pig: 86%; Horse: 100%; Human: 100%; Mouse: 86%; Pig: 100%; Rabbit: 100%; Rat: 86%; Sheep: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

33kDa

References & Citations

Dattani, M.T. et al., (2003) Growth Horm. IGF Res. 16 (9), 1207-1209

Shipping Conditions

Wet Ice

Protein Length

291

NCBI Gene Symbol

POU1F1

NCBI GB Accession Number

POU1F1

Host or Source

Rabbit

Protein Name

Pituitary-specific positive transcription factor 1

Nucleotide Accession Number

NM_000306

Peptide Sequence

TPCLYKFPDHTLSHGFPPIHQPLLAEDPTAADFKQELRRKSKLVEEPIDM

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