E2F4 Antibody - C-terminal region : Biotin (ARP31175_P050-Biotin)

Product Specifications

Gene Name

E2F transcription factor 4, p107/p130-binding

Gene Aliases

E2F-4

Gene ID

1874

Swiss Prot

Q16254

Accession Number

NP_001941

Host

Rabbit

Reactivity

Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Rabbit, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human E2F4

Target

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins. It is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein binds to all three of the tumor suppressor proteins pRB, p107 and p130, but with higher affinity to the last two. It also plays an important role in the suppression of proliferation-associated genes, and its gene mutation and increased expression may be associated with human cancer.

Partner Proteins

RBL2; TFDP1; RBL1; RB1; XPO1; PCM1; HDAC1; POLR1D; CHMP2A; AP3S2; TRIM28; LIN9; ARID3A; NCOR2; HDAC3; AR; CEBPA; LIN54; LIN37; UBC; ATAD2; SIN3A; ASH2L; HCFC1; TRRAP; KAT2A; ID2; E2F1; KMT2A; ID3; SMAD3; NOLC1; TFDP2; NDN; SMAD2; BRCA1; RYBP; RNF144A; FHL

Clonality

Polyclonal

Conjugation

Biotin

Type

Polyclonal Antibody

Applications

IHC, WB

Purification

Affinity Purified

Concentration

0.5 mg/ml

Homology

Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.