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CALR Antibody - C-terminal region : FITC (ARP30114_P050-FITC)

Product Specifications

Gene Name

Calreticulin

Gene Aliases

RO, CRT, SSA, cC1qR, HEL-S-99n

Gene ID

811

Swiss Prot

P27797

Accession Number

NP_004334

Host

Rabbit

Reactivity

Human, Mouse, Rat, Dog, Goat, Guinea Pig, Horse, Rabbit, Yeast, Zebrafish

Immunogen

The immunogen is a synthetic peptide directed towards the C terminal region of human CALR

Target

Calreticulin is a multifunctional protein that acts as a major Ca (2+) -binding (storage) protein in the lumen of the endoplasmic reticulum. It is also found in the nucleus, suggesting that it may have a role in transcription regulation. Calreticulin binds to the synthetic peptide KLGFFKR, which is almost identical to an amino acid sequence in the DNA-binding domain of the superfamily of nuclear receptors. Calreticulin binds to antibodies in certain sera of systemic lupus and Sjogren patients which contain anti-Ro/SSA antibodies, it is highly conserved among species, and it is located in the endoplasmic and sarcoplasmic reticulum where it may bind calcium.

Partner Proteins

HUWE1; UBC; LGR4; MDM2; RNF2; SUZ12; rev; AARSD1; THG1L; NSUN2; SH3GLB1; EEF2K; ARPC1A; ANP32B; ACTR2; ARPC1B; ARPC3; ARPC5; TPD52L2; SH3GL1; RPA3; RPA1; PRKACA; PFDN5; LPP; PARK2; UPF2; UBD; SOX2; PDIA3; CLN5; ITGA3; HLA-E; HLA-A; CALR; SLC27A2; env; MTN

Clonality

Polyclonal

Conjugation

FITC: Fluorescein Isothiocyanate

Type

Polyclonal Antibody

Applications

WB, IHC

Purification

Affinity Purified

Sample Type

CALR is strongly supported by BioGPS gene expression data to be expressed in HepG2

Concentration

0.5 mg/ml

Homology

Dog: 100%; Goat: 86%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 93%; Rat: 100%; Yeast: 92%; Zebrafish: 79%

Format

Liquid. Purified antibody supplied in 1x PBS buffer.

Reconstitution

All conjugated antibodies should be stored in light-protected vials or covered with a light protecting material (i.e. aluminum foil) . Conjugated antibodies are stable for at least 12 months at 4C. If longer storage is desired (24 months), conjugates may be diluted with up to 50% glycerol and stored at -20C to -80C. Freezing and thawing conjugated antibodies will compromise enzyme activity as well as antibody binding.

Molecular Weight

48kDa

References & Citations

Urade, R., et al., (2004) Biochemistry 43 (27), 8858-8868

Shipping Conditions

Wet Ice

Protein Length

417

NCBI Gene Symbol

CALR

NCBI GB Accession Number

CALR

Host or Source

Rabbit

Protein Name

Calreticulin

Nucleotide Accession Number

NM_004343

Peptide Sequence

FGNETWGVTKAAEKQMKDKQDEEQRLKEEEEDKKRKEEEEAEDKEDDEDK

Frequently Asked Questions

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