Anti-Biotin Alexa Fluor® 647
Product Specifications
Certification
RUO
Immunogen
Biotinylated limulus hemocyanin
Target Antigen
Biotin
Clone
1D4-C5
Applications
FC-IC, FC (QC tested)
Concentration
0.1 mg/mL
Format
Alexa Fluor® 647
Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Green NM, Moody KS, Debatis M, Marshak-Rothstein A: Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. J Biol Chem. 2012 Nov 16;287 (47) :39789-99. , URL: https://pubmed.ncbi.nlm.nih.gov/23019335/, *Uccellini MB, Busconi L, Green NM, Busto P, Christensen SR, Shlomchik MJ, Marshak-Rothstein A, Viglianti GA: Autoreactive B cells discriminate CpG-rich and CpG-poor DNA and this response is modulated by IFN-alpha. J Immunol. 2008 Nov 1;181 (9) :5875-84., URL: https://pubmed.ncbi.nlm.nih.gov/18941176/, *Stensland ZC, Smith MJ: Enrichment and detection of antigen-binding B cells for mass cytometry. Magnetochemistry. 2021 Jul;7 (7) :92., URL: https://pubmed.ncbi.nlm.nih.gov/34295938/
Storage Conditions
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Specificity
Mouse monoclonal antibody 1D4-C5 recognizes biotin and can be used as a second-step reagent for detecting biotinylated antigens.
Applications Notes
Flow cytometry: Recomended use: 1 μg/ml. Extracellular and intracellular staining.
Isotype
Mouse IgG2a kappa
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