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Donkey Sheep IgG (H&L), ALP conjugated Antibody

Donkey polyclonal antibody against Donkey anti-Sheep IgG (H&L) conjugated to AP

Product Specifications

Reactivity

Sheep

Immunogen

Purified sheep IgG, whole molecule

Clonality

Polyclonal

Conjugation

ALP

Purification

Immunogen affinity purified donkey IgG.

Dilution

1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA),1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)

Form

Liquid

Storage Conditions

Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Notes

For research use only.

Applications Notes

No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.

Tested Applications

ELISA, ICC, IHC, WB

Host or Source

Donkey

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