Donkey Sheep IgG (H&L), ALP conjugated Antibody
Donkey polyclonal antibody against Donkey anti-Sheep IgG (H&L) conjugated to AP
Product Specifications
Reactivity
Sheep
Immunogen
Purified sheep IgG, whole molecule
Clonality
Polyclonal
Conjugation
ALP
Purification
Immunogen affinity purified donkey IgG.
Dilution
1 : 50-1 : 5000 (ICC), 1 : 500-1 :2000 (ELISA),1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB)
Form
Liquid
Storage Conditions
Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.
Notes
For research use only.
Applications Notes
No reactivity is observed to non-immunoglobulin sheep serum proteins based in immunoelectrophoresis.BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG.
Tested Applications
ELISA, ICC, IHC, WB
Host or Source
Donkey
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