Rat LNGFR (Low Affinity Nerve Growth Factor Receptor) Microsample ELISA Kit

CD271; NGFR; TNFRSF16; P75 (NTR) ; Tumor necrosis factor receptor superfamily member 16; P75 Neurotrophin Receptor; Gp80-LNGFR; Low affinity neurotrophin receptor p75NTR

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LNGFR. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LNGFR. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LNGFR, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LNGFR in the samples is then determined by comparing the OD of the samples to the standard curve.