Rat IL11Ra (Interleukin 11 Receptor Alpha) Microsample ELISA Kit

CRSDA; hCG_2011440; IL-11 receptor subunit alpha; IL-11R subunit alpha; IL-11R-alpha; IL-11RA; IL11RA; Interleukin 11 receptor alpha ; Interleukin 11 receptor alpha chain; Interleukin-11 receptor subunit alpha; MGC2146

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IL11Ra. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IL11Ra. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IL11Ra, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IL11Ra in the samples is then determined by comparing the OD of the samples to the standard curve.