RNase HII (2 U/μL)

RNase HII is a ribonuclease internal enzyme obtained from the Pyrococcus abyssi and recombinantly expressed in E. Coli. It recognizes the DNA-rN-DNA/DNA double strand, cutting at the site where ribonucleotides are incorporated into DNA. However, its activity on single-stranded RNA is very low, and it shows no cutting activity on dsDNA or ssDNA. RNase HII cuts at the site of a single ribonucleotide residue from the 5' end, producing a 5' phosphate group and a 3' hydroxyl end after cutting. This RNase HII enzyme has optimal activity at 70~75°C, is active between 50°C and 75°C, but has very low activity at room temperature. The product is highly thermostable, with almost no loss of activity after incubation at 95°C for 45 minutes, and is compatible with various PCR reaction systems.