Human LILRB4 (Leukocyte Immunoglobulin Like Receptor Subfamily B, Member 4) Microsample ELISA Kit

CD85k; LILR-B4; LILRB5; HM18; ILT3; LIR-5; Immunoglobulin-like transcript 3; Leukocyte immunoglobulin-like receptor 5; CD85 antigen-like family member K

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LILRB4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LILRB4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LILRB4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LILRB4 in the samples is then determined by comparing the OD of the samples to the standard curve.