Rabbit MMP2 (Matrix Metalloproteinase 2) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit MMP2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit MMP2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit MMP2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit MMP2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
CLG4; CLG4A; MMP-II; MONA; TBE-1; PEX; Gelatinase A; 72kDa Gelatinase; 72kDa Type IV Collagenase
UniProt
P50757
Reactivity
Rabbit
Field of Research
Enzyme & Kinase; Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
1.56-100 ng/mL
Sensitivity
0.54 ng/mL
Available Sizes
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