Rat OPN (Osteopontin) ELISA Kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat OPN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat OPN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat OPN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat OPN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Specifications
Product Name Alternative
UniProt
P08721
Reactivity
Rat
Field of Research
Tumor immunity; Bone metabolism
Assay Type
Sandwich
Assay Principle
Assay Performance Time
3.5h
Standard
200 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
3.13-200 ng/mL
Sensitivity
1.31 ng/mL
Available Sizes
Explore Other Products
Browse additional items from our catalog
RUVBL1 (RUVBL1, INO80H, NMP238, TIP49, TIP49A, RuvB-like 1, 49kD TATA box-binding protein-interacting protein, 54kD erythrocyte cytosolic protein, INO80 complex subunit H, Nuclear matrix protein 238, Pontin 52, TIP49a, TIP60-associated protein 54-alpha) (
RUVBL1 (RUVBL1, INO80H, NMP238, TIP49, TIP49A, RuvB-like 1, 49kD TATA box-binding protein-interacting protein, 54kD erythrocyte cytosolic protein, INO80 complex subunit H, Nuclear matrix protein 238, Pontin 52, TIP49a, TIP60-associated protein 54-alpha) (
Rat Monoclonal Aminopeptidase A/ENPEP Antibody (6C3) [PE]
