Mouse SLC7A11 (Solute Carrier Family 7, Member 11) Microsample ELISA Kit

CCBR1; xCT; Cystine/Glutamate Transporter, Cationic Amino Acid Transporter, y+ System; Amino acid transport system xc-; Calcium channel blocker resistance protein CCBR1

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SLC7A11. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SLC7A11. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SLC7A11, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SLC7A11 in the samples is then determined by comparing the OD of the samples to the standard curve.