EasyStep Mouse IGFBP7 (Insulin Like Growth Factor Binding Protein 7) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP7, and the Mouse IGFBP7 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Mouse IGFBP7. After TMB substrate solution is added, only those wells that contain Mouse IGFBP7 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

FSTL2; IGFBP-7v; MAC25; PSF; TAF; IGFBP-rP1; MAC25 protein; Prostacyclin-stimulating factor; PGI2-stimulating factor; Tumor-derived adhesion factor

Reactivity

Mouse

Field of Research

Metabolic pathway

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse IGFBP7, and the Mouse IGFBP7 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells , then added a HRP-conjugated antibody specific to Mouse IGFBP7. After TMB substrate solution is added, only those wells that contain Mouse IGFBP7 and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse IGFBP7 in the samples is then determined by comparing the OD of the samples to the standard curve.