Rat DNM1L (Dynamin 1 Like Protein) ELISA Kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DNM1L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DNM1L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DNM1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DNM1L in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Specifications

Product Name Alternative

DRP1; DLP1; DVLP; DYMPLE; HDYNIV; VPS1; Dynamin-related protein 1; Dnm1p/Vps1p-like protein; Dynamin family member proline-rich carboxyl-terminal domain less

UniProt

O35303

Reactivity

Rat

Field of Research

Infection immunity

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat DNM1L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat DNM1L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat DNM1L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat DNM1L in the samples is then determined by comparing the OD of the samples to the standard curve.