Influenza A virus (Darwin/6/2021) & (Darwin/9/2021) NP (H3N2) Protein, His Tag

Influenza A virus (Darwin/6/2021) & (Darwin/9/2021) NP (H3N2) Protein, His Tag (NP2-V52H3) is expressed from Baculovirus-Insect cells. It contains AA Met 1 - Asn 498 (Accession # EPI1884976, GISAID) .

Product Specifications

Background

Influenza, commonly known as ‘the flu’, is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses.Influenza viral nucleoprotein (NP) is highly conserved and the most abundant non-enzymatic viral protein in infected cells. NP is a key component of the viral ribonucleoproteins (vRNPs) complex, and its recognized functions include, but are not limited to, binding to RNA and oligomerizing for the vRNP complex, undergoing intracellular trafficking, and participating in the switch from mRNA transcription to vRNA replication.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 57.8 kDa. The protein migrates as 55-64 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

EPI1884976

Expression Region

Met 1 - Asn 498

Host

Insect Cells

Target

Nucleocapsid protein/NP (Influenza Virus)

Conjugation

Unconjugated

Tag

C-10xHis

Source

Influenza A virus (Darwin/6/2021) & (Darwin/9/2021)

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Influenza A virus (Darwin/6/2021) & (Darwin/9/2021) NP (H3N2) Protein, His Tag (Cat. No. NP2-V52H3) at 1 μg/mL (100 μL/well) can bind Pan Influenza A Nucleoprotein Antibody, Mouse MAb with a linear range of 0.06-2 ng/mL (QC tested).