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Mouse G-CSF protein, His tag

Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to the IL-6 superfamily.

Product Specifications

Background

Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to the IL-6 superfamily. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent

Product Name Alternative

Granulocyte colony-stimulating factor; Csf3; G-CSF

Gene ID

12985

Accession Number

P09920

Tag

G-CSF

Type

Cytokines and proteins

Applications

His tag

Purity

> 98% as determined by SDS-PAGE

Activity

Measured by its ability to induce proliferation in M-NFS-60 cells. The ED50 for this effect is 1.916 ng/mL.

Molecular Formula

19.8 kDa

Molecular Weight

19.8 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin :< 0.1 EU per μg of the protein as determined by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized recombinant Mouse G-CSF protein product should be stored desiccated below -20°C.Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Mouse G-CSF consists of 178 amino acids and predicts a molecular mass of 19.8 kDa.

Formulation

Lyophilized from sterile 20mM Tris, 150mM NaCl, 5% Sucrose, 5% Mannitol, pH9.0.

Applications Notes

Mouse

Other Product Names

Granulocyte colony-stimulating factor; Csf3; G-CSF

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Mouse G-CSF protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions

Preparation

E.coli

Accession Number URL

Csf3 - Granulocyte colony-stimulating factor - Mus musculus (Mouse) | UniProtKB | UniProt

AA Sequence

Amino acid sequence derived from Mouse G-CSF (Val31-Ala208) (P09920) was expressed with 6xHis tag.

Available Sizes

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