Alamar Blue

Alamar Blue is an oxidative reducing indicator that can generate changes and fluorescent signals according to metabolic activity. This is a safe and non -toxic dye that can be used for quantitative analysis of cell activity and cell proliferation as well as cytokine biological determination and in vitro cytotoxicity. It can effectively determine the natural metabolic activity of animals, fungi and bacterial cells. Alamar Blue is purple -blue without fluorescence in the oxidation state. In the restore state, it transforms into a pink or red fluorescent restoration product, reflecting the consumption of oxygen molecules studied by the cells or microorganisms studied, so it is often used as oxidation. Restore the indicator to show metabolic activities observed cells and bacteria. This determination is the ability to convert reagents into fluorescence and color -color indicator based on metabolic activity. During the process of cell proliferation, the ratio of NADPH/NADP, FADH/FAD, FMNH/FMN, and NADH/NAD increased in the cells. The dyes intake in the cells are restored by these metabolic intermediates and cytochromes and release them to the outer cells and dissolved in the medium, so that the medium will change from a fluorescent blue blue to fluorescent pink. Damage and active cells have low natural metabolic activity, so the corresponding signals are lower. It can be detected with ordinary optical light meter or fluorescent light meter. It stimulates the wavelength between 530-560 nm and the transmitting light wavelength is 590 nm. Inhalery and fluorescent strength are directly proportional to the number of active cells.