Recombinant Human LIF

Product Specifications

Product Name Alternative

CDF, Cholinergic Differentiation Factor, D factor, DIA, Differentiation inducing factor, differentiation inhibitory activity, Differentiation stimulating factor

UniProt

P15018

Expression Region

23-202

Host

E.Coli

Tag

N-terminal His Tag

Applications

Western Blot, ELISA

Field of Research

Signal Transduction, Immunology, Developmental biology, Neuroscience, Stem Cells

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 0.04-0.10 ng/mL.

Form

Lyophilized powder

Reconstitution

Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.

Molecular Weight

21.6 kDa

Shipping Conditions

4°C with ice bag

Storage Conditions

The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.