Recombinant Human LIF
Product Specifications
Product Name Alternative
CDF, Cholinergic Differentiation Factor, D factor, DIA, Differentiation inducing factor, differentiation inhibitory activity, Differentiation stimulating factor
UniProt
P15018
Expression Region
23-202
Host
E.Coli
Tag
N-terminal His Tag
Applications
Western Blot, ELISA
Field of Research
Signal Transduction, Immunology, Developmental biology, Neuroscience, Stem Cells
Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.The ED50 for this effect is 0.04-0.10 ng/mL.
Form
Lyophilized powder
Reconstitution
Centrifuge the vial before opening, reconstitute in sterile distilled water to a concentration of 0.1-1 mg/ml by gently pipetting 2-3 times, don't vortex.
Molecular Weight
21.6 kDa
Shipping Conditions
4°C with ice bag
Storage Conditions
The lyophilized protein is stable at -20 °C for up to 1 year. For extended storage, it is recommended to further dilute in working aliquots after reconstitution. The protein solution is stable at ≤ -20 °C for 3 months, or 2-7 days at 2-8 °C under sterile conditions.Avoid repeated freeze/thaw cycle.
Formulation
Lyophilized from a 0.2 μm filtered solution of 10 mM Hepes, 150 mM NaCl with 5% trehalose, pH 7.4.
Organism Species
Human
Available Sizes
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