Ac-Ile-Glu-Thr-Asp-pNA

Ac-Ile-Glu-Thr-Asp-pNA is a substrate for caspase-8. Caspase-8 binds to and cleaves the Ile-Glu-Thr-Asp (IETD) peptide sequence to release p-nitroalinide, which can be quantified by colorimetric detection at 405 nm as a measure of enzyme activity[1].

Product Specifications

CAS Number

[219138-21-3]

UNSPSC

12352209

Target

Caspase

Type

Peptides

Related Pathways

Apoptosis

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/ac-ile-glu-thr-asp-pna.html

Purity

99.90

Solubility

1 M NaOH : 3.33 mg/mL (ultrasonic; warming; heat to 60°C) |H2O : 0.98 mg/mL (adjust pH to 12 with 1 M NaOH)

Smiles

O=C(N[C@@H]([C@H](O)C)C(N[C@@H](CC(O)=O)C(NC1=CC=C([N+]([O-])=O)C=C1)=O)=O)[C@H](CCC(O)=O)NC([C@@H](NC(C)=O)[C@@H](C)CC)=O

Molecular Formula

C27H38N6O12

Molecular Weight

638.62

References & Citations

[1]Smith GK, et al. Expression, preparation, and high-throughput screening of caspase-8: discovery of redox-based and steroid diacid inhibition. Arch Biochem Biophys. 2002;399 (2) :195-205.