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Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag

Selected Variant

PON-V51H3-1mg

Size: 1 mg

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

Product Specifications

Background

Plays an essential role in viral RNA transcription and replication by forming the heterotrimeric polymerase complex together with PB1 and PB2 subunits. The complex transcribes viral mRNAs by using a unique mechanism called cap-snatching. It consists in the hijacking and cleavage of host capped pre-mRNAs. These short capped RNAs are then used as primers for viral mRNAs. The PB2 subunit is responsible for the binding of the 5' cap of cellular pre-mRNAs which are subsequently cleaved after 10-13 nucleotides by the PA subunit that carries the endonuclease activity.

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 26.5 kDa. The protein migrates as 28-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under non-reducing (NR) condition (SDS-PAGE) .

Accession Number

P31343-1

Expression Region

Met 1 - Gly 209

Host

E. coli

Target

Polymerase acidic protein

Conjugation

Unconjugated

Tag

C-10xHis

Source

Influenza A virus (strain A/Victoria/3/1975 H3N2)

Stability

The product MUST be stored at -70°C or lower upon receipt; -70°C for 3 months under sterile conditions.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Influenza A virus (strain A/Victoria/3/1975 H3N2) Polymerase acidic Protein, His Tag (Cat. No. PON-V51H3) at 1 μg/mL (100 μL/well) can bind Influenza A PA Polyclonal Antibody,Rabbit/IgG with a linear range of 0.2-16 ng/mL (QC tested).

Format

Liquid

Buffer

PBS, 0.5 M Arginine, 50% Glycerol, pH7.4

Additives

Trehalose

Molecular Weight

26.5 kDa

Additionnal Information

Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/support/shipping-and-payments

Shipping Conditions

Dry ice

Storage Conditions

-70°C

Package Size

1mg*1

Host or Source

E. coli

Species

Influenza A virus (strain A/Victoria/3/1975 H3N2)

Protein ID

P31343-1

Preservative

Trehalose

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