Sulfo-SANPAH

Sulfo-SANPAH is a primary amine-nitrobenzene azide cross-linker. Sulfo-SANPAH improves the functionalization process of PDMS surfaces, is covalently bound to the PAAm gel surface. Sulfo-SANPAH is widely used to crosslink ECM proteins to various substrates, including acrylic-based hydrogels, such as polyacrylamide hydrogels. Sulfo-SANPAH facilitates covalent binding through its negatively charged sulfonate group on its N-hydroxysuccinimide ester ring and a photoactivated phenyl azide group that is highly reactive with nucleophiles and free radicals[1][2].

Product Specifications

CAS Number

[102568-43-4]

UNSPSC

12352005

Hazard Statement

H302+H312+H332, H315, H319, H335

Target

Biochemical Assay Reagents; Reactive Oxygen Species (ROS)

Type

Reference compound

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Others

Applications

Neuroscience-Neuromodulation

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/sulfo-sanpah.html

Purity

99.75

Solubility

1 M NaOH : 25 mg/mL (ultrasonic; warming; heat to 60°C) |DMSO : 125 mg/mL (ultrasonic)

Smiles

O=C(ON1C(C(S(=O)(O)=O)CC1=O)=O)CCCCCNC2=CC=C(N=[N+]=[N-])C=C2[N+]([O-])=O

Molecular Formula

C16H18N6O9S

Molecular Weight

470.41

Precautions

H302+H312+H332, H315, H319, H335

References & Citations

[1]Vázquez-Victorio G, et al. Fabrication of Adhesive Substrate for Incorporating Hydrogels to Investigate the Influence of Stiffness on Cancer Cell Behavior. Methods Mol Biol. 2021;2174:277-297.|[2]Pérez-Calixto, M., et al., (2025) . Enhanced PDMS Functionalization for Organ-on-a-Chip Platforms Using Ozone and Sulfo-SANPAH: A Simple Approach for Biomimetic Long-Term Cell Cultures. Advanced healthcare materials, 14 (13), e2404686. Advance online publication.|[3]Home A. 2023 AIChE Annual Meeting (27bv) Investigating the Effects of Surface Stiffness on Human Mesenchymal Stem Cell Immunomodulatory Potential[J].