Recombinant Human IFN gamma (E. coli)
Recombinant Human Interferon Gamma is produced by our E.coli expression system and the target gene encoding Gln24-Gln166 is expressed.
Product Specifications
Background
IFNγ is the major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly byT-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFNγ synthesis is induced by IL-2, FGF-basic, and EGF.
Synonyms
Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG
UniProt
P01579
Accession Number
P01579
Endotoxin
Less than 0.001 ng/µg (0.01 EU/µg) as determined by LAL test.
Purity
Greater than 95% as determined by reducing SDS-PAGE.
Bioactivity
Measured by a cytotoxicity assay using HT-29 cells. The ED50 for this effect is 17 pg/ml.
Reconstitution
Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Molecular Weight
16.88 KDa
Shipping Conditions
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Storage Conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Notes
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Observed Molecular Weight
16 KDa, reducing conditions
Other Product Names
Interferon Gamma; IFN-Gamma; Immune Interferon; IFNG
CAS Number
9000-83-3
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