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Recombinant Human/Mouse FGF-8b

Recombinant Human/Mouse Fibroblast Growth Factor 8B is produced by our E.coli expression system and the target gene encoding Gln23-Arg215 is expressed.

Product Specifications

Background

Fibroblast growth factor 8 (FGF­8) is a member of the fibroblast growth factor family. It is discovered as a growth factor essential for the androgen-­dependent growth of mouse mammary carcinoma cells. Mouse FGF­8b shares 100% aa identity with human FGF­8b. FGF­8 is widely expressed during embryogenesis, and mediates epithelial­-mesenchymal transitions. It plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. It is required for normal brain, eye, ear, limb development during embryogenesis and normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.

UniProt

P55075-3/P37237-2

Accession Number

P55075-3/P37237-2

Endotoxin

Less than 0.001 ng/μg (0.01 EU/μg) as determined by LAL test.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Bioactivity

Measured in a cell proliferation assay using BALB/c 3T3 cells. The ED50 for this effect is 21.87 ng/ml.

Molecular Weight

22.5 KDa

Shipping Conditions

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Temperature

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Notes

Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Observed Molecular Weight

23 KDa, reducing conditions

Other Product Names

Fibroblast growth factor 8; Androgen-induced growth factor; Heparin-binding growth factor 8; AIGF; HBGF-8; FGF-8B

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