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Recombinant 2019-nCoV NSP10 (N-6His)

Recombinant 2019-nCoV NSP10 is produced by our E.coli expression system and the target gene encoding Ala1-Gln139 is expressed with a 6His tag at the N-terminus.

Product Specifications

Background

Nsp10 have shown that it is a 15-kDa protein of unknown function that has been shown to interact with itself, nsp1, and nsp7. It colocalizes with N to sites of viral replication and is essential for replication. It plays a pivotal role in viral transcription by stimulating both nsp14 3'-5' exoribonuclease and nsp16 2'-O-methyltransferase activities. Therefore plays an essential role in viral mRNAs cap methylation. Nsp10 is a critical regulator of coronavirus RNA synthesis and may play an important role in polyprotein processing.

UniProt

YP_009725306.1

Accession Number

YP_009725306.1

Purity

Greater than 80% as determined by reducing SDS-PAGE.

Molecular Weight

17.9 KDa

Shipping Conditions

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Storage Temperature

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.

Notes

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Observed Molecular Weight

18 KDa, reducing conditions

Other Product Names

SARS-CoV 2 nsp10; SARS-CoV 2 Growth factor-like peptide; SARS-CoV 2 GFL

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